Composite

Part:BBa_K5416030:Design

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-29)


PhaP PhaC-HRT2trunc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Condon optimized for expression in E. coli, all RBS sequences are optimized for expression in E. coli strain K-12 specific to this part.


Source

Sourced from BBa_K208001; BBa_K5416000 and reported amino acid sequence of PhaC from C. necator (Uniprot P23608). The entire part is synthesized through de novo dna synthesis

References